農業科學  >> Vol. 2 No. 3 (September 2012)

優良品種龍灘珍珠李自花結實分子機理初探
Pre-Study on the Molecular Mechanism of Self-Compatibility in Longtanzhenzhuli

DOI: 10.12677/HJAS.2012.23009, PDF, HTML, XML, 下載: 2,997  瀏覽: 9,582 

作者: 葉燕萍, 孟麗萍:廣西大學農學院;黃紹西:廣西農業科學院園藝研究所

關鍵詞: 龍灘珍珠李自交不親和自花結實S-RNase(S)基因Longtanzhenzhuli; Self-Incompatibility; Self-Compatibility; S-Rnase Genes

摘要: 本研究主要以廣西本地生長的優質的具自花結實李品種龍灘珍珠李為材料,根據李屬雌蕊S-RNase基因的序列信息設計了一對特異性保守引物,對龍灘珍珠李基因組DNA進行PCR擴增,并對擴增產物進行克隆、測序以及序列分析,成功克隆出兩個S-RNase基因片段,其中一個為在中國李中首次發現并命名為PsS-RNase-28,大小為727 bp,其中包含有367 bp的內含子,僅有360 bp的外顯子編碼序列,與黑刺李(Prunus spinosa)S-RNase S1基因(EF636467.1)同源率達到98%,與其它李S- RNase基因的同源率都達到79%以上。另一個擴增片段大小為718 bp,包含有343 bp的內含子,其中僅有375 bp的外顯子編碼序列,與中國李(Prunus salicina)PsS-RNase-h基因(AB084148.1)相似性達到99%,與其它李S-RNase基因的相似性都達到85%以上。這兩個基因片段的獲得及其后續分析有利于進一步探討龍灘珍珠李自花結實的分子機理。
Abstract: Longtanzhenzhuli which is a new variety of plum with high quality were used as the experimental materials in this research. A specific primer was designed based on the conserved sequences of plum (Prunus salicina Lindl.) S-Rnase(S) genes, two specific fragments were isolated from Longtanzhenzhuli genome DNA with tese specific primers. The sequencing results showed that these fragments were confirmed as the plum S genes. One was first cloned from Prunus salicina and named PsS-RNase-28, with length of 727 bp, containing 367 bp intron and 360 bp Exton. Comparing with Prunus spinosa S-RNase S1 gene (EF636467.1), it showed high identity (98%), more than 79% with other plums S-RNase genes. The other fragment with 718 bp in length, containing 343 bp intron and 375 bp Exton, showed high identity (99%) with Prunus salicina PsS-RNase-h gene (AB084148.1), and more than 85% with other plums S-RNase genes. And sequences follow-up analysis of these fragments would be benefited to study on the mechanism of self-compatibility of Longtanzhenzhuli.

文章引用: 葉燕萍, 黃紹西, 孟麗萍. 優良品種龍灘珍珠李自花結實分子機理初探[J]. 農業科學, 2012, 2(3): 49-57. http://dx.doi.org/10.12677/HJAS.2012.23009

參考文獻

[1] 陳迪新, 張紹鈴, 陶書田. 沙梨花粉原位萌發與花粉管生長特性[J]. 南京農業大學學報, 2004, 27(3): 34-37.
[2] 李曉, 吳華清, 吳俊, 陶書田, 張紹鈴. 不同發育階段甜櫻桃花授粉后花粉萌發和花粉管生長的差異[J]. 果樹學報, 2008, 25(4): 501-505.
[3] 李曉芳, 李茂福, 韓振海, 許雪峰, 李天忠. “鴨梨”芽變“閆莊梨”自交親和性分子機制初步研究[J]. 園藝學報, 2007, 35(1): 13-18.
[4] 吳華清, 齊永杰, 張紹鈴. “奧嗄二十世紀”梨自交親和性分子機制及其遺傳特性研究[J]. 園藝學報, 2008, 35(8): 1009-1116.
[5] 吳華清, 衡偉, 李曉, 黃紹西, 張紹鈴. “大果黃花”梨自交親和性變異機制研究[J]. 南京農業大學學報, 2007, 30(2): 29-33.
[6] R. Tao, T. Habu, A. Namba, H. Yamane, F. Fuyuhiro, K. Iwamoto and A. Sugiura. Inheritance of Sf-RNase in Japanese apricot (Prunus mume) and its relation to self-compatibility. Theoretical and Applied Genetics, 2002, 105: 222-228.
[7] K. Ushijima, H. Yamane, A. Watari, E. Kakehi, K. Ikeda, N. R. Hauck, A. F. Iezzoni and R. Tao. The S haplotype-specific F-box protein gene, SFB, is defective in self-compatible haplotypes of Prunus avium and P. mume. Plant Journal, 2004, 39: 573-586.
[8] S. Vilanova, M. L. Badenes, L. Burgos, J. Martinez-Calvo, G. Llacer, and C. Romero. Self-compatibility of two apricot selections is associated with two pollen-part mutations of different nature. Plant Physiology, 2006, 142: 629-641.
[9] W. Broothaerts, J. Keumans and I. V. Nerum. Self-fertile apple resulting from S-RNase gene silencing. Plant Cell Reports, 2004, 22: 497-501.
[10] 黃紹西. 中國李(Prunus salicina)自交不親和性與中國櫻桃(P. pseudocerasus)自交親和性機制的初步研究[D]. 南京: 南京農業大學, 2009.
[11] Y. Kawata, et al. Amino-acid sequence of ribonucease T2 from Aspergillus oryzae. European Journal of Biochem, 1988, 103: 408-418.
[12] B. A. Mcclure, V. Haring, P. R. Ebert, et al. Style self-income- patibility gene products of nicotiana alata are ribonucleases. Nature, 1989, 342: 955-957.
[13] T. R. Ioerger, A. G. Clark, T.-H. Kao. Polymorphism at the self-incompatibility locus in Solanaceae predates speciation. Proc Natl Acad Sci USA, 1990, 87: 732-735.
[14] A. D. Richman, W. Broothaerts, J. R. Kohn. Self-incompatibility Rnase from the three plant families: Homology or convergence. Am J Bot, 1997, 84: 912-917.
[15] R. Tao, H. Yamane, H. Sassa, et al. Identification of stylar RNase associated with gametophytic self-incompatibility in almond (Pyrus dulcis). Plant Cell Physiology, 1997, 38: 304-311.
[16] 張樹軍, 黃紹西, 張紹鈴等. 攜李等15個李品種S基因型鑒定及其多態性分析[J]. 果樹學報, 2008, 25(3): 338-342.

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